Table 2 Results from the mixed-model nested ANOVAs on differentiation of life-history traits of male Poecilia mexicana.

From: Sex-specific local life-history adaptation in surface- and cave-dwelling Atlantic mollies (Poecilia mexicana)

Trait

Ecotype

Light regime

Food regime

Growth rate [mm/d]

χ 2  = 14.114, P  = 0.0002

χ 2  = 3.939, P  = 0.047

χ 2  = 6.981, P  = 0.0082

Maturation time [d]

χ2 = 1.451, P = 0.23

χ2 = 0.005, P = 0.95

χ 2  = 5.907, P  = 0.015

Age at maturity [d]

χ 2  = 149.991, P  < 0.0001

χ2 = 2.075, P = 0.15

χ 2  = 19.475, P  < 0.0001

SL at maturity [mm]

χ 2  = 7.782, P  = 0.0053

χ 2  = 15.849, P  < 0.0001

χ 2  = 18.741, P  < 0.0001

Lean weight at maturity [g]

χ 2  = 4.483, P  = 0.034

χ2 = 0.944, P = 0.33

χ2 = 0.018, P = 0.89

Fat content at maturity [%]

χ2 = 2.389, P = 0.12

χ 2  = 8.577, P  = 0.0034

χ 2  = 34.922, P  < 0.0001

GSI at maturity [%]

χ2 = 1.153, P = 0.28

χ2 = 0.537, P = 0.46

χ2 = 0.290, P = 0.59

Trait

Ecotype × Light regime

Ecotype × Food regime

Light regime × Food regime

Growth rate [mm/d]

(χ2 = 0.358, P = 0.55)

(χ2 = 1.147, P = 0.28)

(χ2 = 0.115, P = 0.73)

Maturation time [d]

(χ2 = 0.013, P = 0.91)

χ 2  = 4.196, P  = 0.041

(χ2 = 1.299, P = 0.25)

Age at maturity [d]

(χ2 = 1.165, P = 0.28)

χ 2  = 11.468, P  = 0.0007

(χ2 = 0.095, P = 0.76)

SL at maturity [mm]

(χ2 = 1.507, P = 0.22)

(χ2 = 0.107, P = 0.74)

χ2 = 2.862, P = 0.091

Lean weight at maturity [g]

(χ2 = 0.394, P = 0.53)

(χ2 = 0.562, P = 0.45)

(χ2 = 0.622, P = 0.43)

Fat content at maturity [%]

(χ2 = 1.044, P = 0.31)

(χ2 = 0.734, P = 0.39)

χ2 = 2.846, P = 0.092

GSI at maturity [%]

(χ2 = 0.714, P = 0.40)

(χ2 = 0.028, P = 0.87)

(χ2 = 2.345, P = 0.13)

  1. Fixed effects were ‘ecotype (cave vs. surface)’, ‘light regime (dark vs. light:dark)’ and ‘food regime (high vs. low)’, while ‘mother ID’ and ‘block(room)’ were included as random effects. Significant test statistics are in bold and test statistics in parentheses refer to non-significant interactions that were removed from the final model.