Figure 3
From: Pneumococcal Pneumolysin Induces DNA Damage and Cell Cycle Arrest
Pneumolysin-induced DNA damage is associated with cell cycle arrest.
(A) Alveolar epithelial cells exposed to pneumolysin were lysed at different time-points during incubation and the lysate was probed for p53 and p21 by western blot. β-actin served as loading control. Each blot is representative of three independent experiments. Protein band intensity analysis was done on all three experiments and is expressed as fold change relative to ‘media only’ control after normalizing with β-actin. (B) Cells exposed to pneumolysin were also analyzed for cell cycle using propidium iodide. Representative histograms of three independent experiments showing alveolar epithelial cell arrest at 4N DNA content after treatment with pneumolysin for 48 h. Percentage cell cycle arrest at 12, 24 and 48 h was quantified. Results show mean ± SEM for three independent experiments. *p < 0.05, unpaired Student’s t-test.
