Figure 4
From: Pneumococcal Pneumolysin Induces DNA Damage and Cell Cycle Arrest
Association between pneumolysin-induced γH2AX and DNA replication.
(A) Alveolar cells incubated with pneumolysin for 4, 12 and 48 h were labeled with EdU for 30 min at the end of each time point. The cells were then fixed and probed for γH2AX and EdU. Representative images showing nuclei (blue), γH2AX (red) and EdU (yellow). Images are representative from three independent experiments. (B) γH2AX, EdU positive (≥5 foci per nucleus) and dual-positive cells were quantified and expressed as percentage positive cells. ‘Media only’ indicates negative control without pneumolysin. Results show mean ± SEM for three independent experiments.
