Figure 3: Phylogenetic relationships, gene structure and motif compositions of the Gossypium raimondii R2R3-MYB genes.

(A) The phylogenetic tree was constructed with MEGA 5.0 using the Neighbor‐Joining (NJ) method with 1,000 bootstrap replicates based on a multiple alignment of 205 amino acid sequences of R2R3-MYB genes from G. raimondii. Bootstrap values higher than 50% are shown on the nodes. The 50 major subfamilies are indicated with C1 to C0 are marked with colorful backgrounds. (B) Exon/intron structures of R2R3-MYB genes from G. raimondii. The exons and introns are represented by green boxes and black lines, respectively. The scale bar represents 1.0 kb.(C) Protein motif. Schematic diagram of the conserved motifs in the R2R3-MYB proteins in cotton, which were elucidated using MEME. Each motif is represented by a number in the colored box. The black lines represent the non-conserved sequences. The scale bar represents 200 aa.