Figure 4: hnRNPK inhibited GSK3β Ser9 phosphorylation by PKC. | Scientific Reports

Figure 4: hnRNPK inhibited GSK3β Ser9 phosphorylation by PKC.

From: hnRNPK inhibits GSK3β Ser9 phosphorylation, thereby stabilizing c-FLIP and contributes to TRAIL resistance in H1299 lung adenocarcinoma cells

Figure 4

H1299 cells respectively transfected with Flag-hnRNPK (a) or hnRNPK siRNA (b), and treated with or without TRAIL (20 ng/ml, 8 hours) were harvested for Western blot analysis with the indicated antibodies. (c) PKC inhibitor Gö6983 cancelled the regulatory effect of hnRNPK on GSK3β Ser9 phosphorylation. H1299 cells transfected with hnRNPK siRNA or/and treated with Gö6983 (1 μM) were analyzed by Western blotting with the indicated antibodies. (d) PKC inhibitor Rottlerin had no obvious effect on the regulation of GSK3β Ser9 phosphorylation by hnRNPK. H1299 cells transfected with hnRNPK siRNA or/and treated with Rottlerin (6 μM) were analyzed by Western blotting with the indicated antibodies. (e) PP1 inhibitor Okadaic acid did not affect the regulation of GSK3β Ser9 phosphorylation by hnRNPK. H1299 cells transfected with Flag-hnRNPK or/and treated with okadaic acid (100 nM) were analyzed by Western blotting with the indicated antibodies.

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