Table 2 Biochemical parameter in blood, plasma, feces and liver (experiments 1–3).

From: Castration influences intestinal microflora and induces abdominal obesity in high-fat diet-fed mice

 

SD

HFD

HFD + Antibiotics

Sham

Castration

Sham

Castration

Sham

Castration

Blood

 Glucose (mg/dl)

82.5 ± 5.0

94.7 ± 5.8

94.1 ± 3.6

133.7 ± 6.0*

86.7 ± 4.8

99.6 ± 7.3

Plasma

 Insulin (ng/ml)

1.4 ± 0.3

0.5 ± 0.2*

2.5 ± 0.5

1.8 ± 0.3

1.5 ± 0.5

1.0 ± 0.2

 Free fatty acid (mM)

1.33 ± 0.09

1.33 ± 0.10

1.04 ± 0.06

1.03 ± 0.05

4.39 ± 0.19

3.92 ± 0.16

 Triglyceride (mg/dl)

135 ± 14

86 ± 10*

79 ± 11

70 ± 9

52 ± 7.0

49 ± 4.0

Feces

 Triglyceride (mg/g)

10.7 ± 0.4

10.2 ± 0.5

11.2 ± 0.4

10.7 ± 0.4

5.4 ± 1.0

5.3 ± 0.7

 Total Cholesterol (mg/g)

5.2 ± 0.6

5.3 ± 0.2

6.8 ± 0.7

3.7 ± 0.2*

4.9 ± 0.4

3.4 ± 0.3*

Liver

 Triglyceride (mg/g)

30.0 ± 3.9

39.2 ± 9.0

49.1 ± 5.9

92.3 ± 9.6*

151 ± 29

177 ± 10

 Total Cholesterol (mg/g)

4.4 ± 0.3

5.3 ± 0.6

6.0 ± 0.3

6.4 ± 0.3

13.7 ± 1.2

14.2 ± 0.5

  1. Data are expressed as means ± SEM. Statistical differences (*p < 0.05) were determined between sham-operated mice and castrated mice. Blood glucose levels and feces were analyzed at 21 and 17 weeks, respectively. Plasma and liver were analyzed after dissection.