Table 3 Technical Comparison between different methods and MTT based method.

From: Colorimetric method for rapid detection of Oxacillin resistance in Staphylococcus aureus and its comparison with PCR for mec A gene

Method

Media requirement per test

Time required

MIC determination

Complexity

Agar dilution Method 9

300 ml

24 h

Yes

More tedious with higher media requirement

Easy MIC Test 9

20 ml

24 h

Yes

Required Easy MIC strips which increased the cost

MTT based Method

300 μl

7 h

Yes

Very less media required, rapid and simple

Oxacillin Screen Agar 9

20 ml

24 h

No

Cannot detect borderline resistant strains

Oxacillin Disc Diffusion 9

20 ml

24 h

No

Gives false positive results

PCR for mec A 14

12–16 h

No

Set up not available in all laboratories