Figure 1: Myeloid PTEN deficiency exacerbates BLM induced pulmonary fibrosis. | Scientific Reports

Figure 1: Myeloid PTEN deficiency exacerbates BLM induced pulmonary fibrosis.

From: Sustained PI3K Activation exacerbates BLM-induced Lung Fibrosis via activation of pro-inflammatory and pro-fibrotic pathways

Figure 1

Myeloid PTEN deficient mice and wild-type littermates were treated with BLM (0.1 iu) intra-nasally. (a) The weight loss was assessed daily. Data are presented as mean +/− SEM and analysed by 2-way ANOVA, n = 19–20, ****p < 0.0001. (b) Myeloid PTEN deficiency led to a decreased survival. The mice were monitored over 21 days and the Kaplan Meier test was used for statistics, n = 20–24, *p < 0.05. (c) Hydroxyproline was quantified as measure for the collagen content in the lung after 21d and was statistically analysed by the Mann Whitney test, n = 4–5. (d) Representative images of healthy control lungs (labelled in gray) and BLM treated lungs (labelled in black) of a wild-type and a myeloid PTEN deficient mice (from left to right, 200× (large images) and 630× (small images) magnification). The lungs were stained with hematoxylin and eosin, Sirius red (collagen fibres stained red) and Masson’s Trichrome stain(muscle fibers = red, erythrocytes = orange, collagen = green) (top-down), scale bars indicate 75 μm or 25 μm in the 200× or in the 630× magnification images, respectively. (e) The level of pulmonary fibrosis was scored according to the Sirius red staining of the histological lung specimen, n = 4–5.

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