Figure 6: Pro-inflammatory cytokines are increased and macrophages display a shift towards an M1 phenotype.

The cytokines IL-6 (a), IL-10 (b), IL-17A (c), IL-22/23 (d), IL-13 (e), IL-4 (f), INF-γ (g) and TNF-α (h) were measured by ELISA in homogenized lung tissue from wild-type and myeloid PTEN deficient mice at 7d post BLM treatment. For statistical analysis the unpaired Student’s t-test (b,d) or the Mann Whitney test (a,c,e,h) was performed, n ≥ 9, *p < 0.05. (i,j) Lungs from mice subjected to pulmonary fibrosis were digested by DNAse I and Collagenase I to generate a single cell suspension. Macrophages were identified by CD45⁺ and F4/80⁺ and further analysed for their expression of CD80, CD86 (i) and iNOS, ArgI (j). For statistical analysis the unpaired Student’s t-test was performed, n ≥ 5, *p < 0.05, **p < 0.01.