Figure 1
From: Mitochondrial Thioredoxin System as a Modulator of Cyclophilin D Redox State
PPIase activity of CypD in the mitochondrial matrix.
Mitochondrial matrix (30 μg proteins) was preincubated in 100 mM NaCl, 50 mM HEPES/Tris (pH 8.0) at 25 °C for 15 min in the presence, where indicated, of 300 μM NADPH, 0.4 μM TrxR1, 3 μM Trx from E. coli or 1 μM auranofin (AF). PPIase activity was estimated by a coupled assay utilizing α-chymotrypsin as described under Methods. (A) time course of PPIase activity in different experimental conditions and (B) first-order rate constants (s-1) indicated in the ordinate axis (*p < 0.01) and corresponding to reactions obtained in conditions similar to those of the curves reported in (A). Mean ± SD, n = 5 are shown.
