Figure 3: Detection of apoptosis by fluoresence microscopy in cells after treatment with AHC compounds.

(a) PC-3 cells were treated with GC, CG or MG for 48 h, as indicated below the panels. Cells were stained with DAPI, acridine orange and ethidium bromide (AO/EB), or propidium iodide (PI) as indicated on the right side of the panels. (b) MDA-MB-231 cells were treated with CG or MG for 48 h as indicated below the panels. Cells were stained with DAPI, acridine orange and ethidium bromide (AO/EB), or propidium iodide (PI) as indicated on the right of the panels. Images of cells were captured at 200-fold magnification. Scale bar is 100 μm.