Figure 2
From: Identification of early myeloid progenitors as immunosuppressive cells
Potent suppression of T cell proliferation by early myeloid progenitor cells.
(A,C) Inhibition of non-specific T cell proliferation by GMPs and CMPs. 2 × 105 CFSE-labeled B6 splenocytes were cultured with or without indicated BM populations at a 1:1 (A) or 1:8 (C) ratio of BM cells vs splenocytes for 3 days in the presence of anti-CD3/anti-CD28 antibodies. (B,D) Inhibition of antigen-specific T cell proliferation by GMPs and CMPs. Cell culture procedure was similar to that as in Fig. 1A, except that mitomycin C-treated BALB/c splenocytes (2 × 105) instead of anti-CD3/anti-CD28 antibodies were used as stimulators and cells incubated for 96 hrs. Data shown are mean ± SD of triplicate samples and representative of at least five experiments each performed with pooled samples from 4–5 mice per group. ***p < 0.001; **p < 0.01; *p < 0.05.
