Figure 2 | Scientific Reports

Figure 2

From: Single-molecule analysis of DNA replication reveals novel features in the divergent eukaryotes Leishmania and Trypanosoma brucei versus mammalian cells

Figure 2

Single-molecule analysis of the inter-origin distances in trypanosomatids using molecular combing.

(A) Representative chromosome fibres from trypanosomatids used for the analysis of inter-origin distances (IOD). Positions of the presumed origins are indicated with arrows. Scale bar: 50 kb. For each species, upper panels: counterstaining of DNA fibres is in blue, first pulse nucleosides (IdU) in red and second pulse nucleosides (CldU) in green; lower panels show the tracks from the first and second pulse nucleosides extracted from the upper panel. (B) Statistical distribution (frequency) of IODs in MEF cells and four trypanosomatids. (C) Comparative analysis of IODs in MEF cells and four trypanosomatids obtained after one-way ANOVA analysis of variance. Boxes present 25–75% range. Whiskers present minimum–maximum range. The two-tailed Mann-Whitney test was used to compare the median values of IOD between each two samples and to calculate the corresponding P values (P < 0.05 was taken as significant).

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