Figure 5 | Scientific Reports

Figure 5

From: Single-molecule analysis of DNA replication reveals novel features in the divergent eukaryotes Leishmania and Trypanosoma brucei versus mammalian cells

Figure 5

Analysis of synchronous and asynchronous adjacent origin firing.

(A) Representative chromosome fibres used for the analysis of DNA replication dynamics showing synchronous (upper picture) and asynchronous (lower picture) firing of adjacent origins (arrows). Diagrams: “a” and “b” represent the lengths of the tracks of non-labelled DNA in two adjacent origins. They are used to discriminate between synchronous and asynchronous origins: if “a = b” the two adjacent origins are synchronous, if “a ≠ b”, they are asynchronous. (B) The number of DNA fibres bearing synchronous and asynchronous adjacent origin firing was counted in the five cell types (MEF cells, T. brucei and three Leishmania species). Numbers and percentages are presented in the table (right) and the percentages of each type of firing represented as histograms (left). The Chi-square test was used to calculate the P values (P < 0.05 was taken as significant).

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