Table 1 DNA replication parameters in three Leishmania species, T. brucei and MEF cells.
L. donovani | L. major | L. mexicana | T. brucei | MEF cells | |
|---|---|---|---|---|---|
Inter-origin distance (kb) | |||||
Median (kb) | 179.7 | 192.7 | 203.3 | 148.8 | 124.6 |
Mean (kb) | 188.5 | 194.8 | 226 | 159.9 | 135.8 |
Minimum (kb) | 29.5 | 48.6 | 49.3 | 36.7 | 27.4 |
Maximum (kb) | 628.1 | 625.4 | 628.1 | 421.6 | 372.3 |
25% Percentile (kb) | 114.2 | 128.2 | 140.7 | 103.8 | 86.37 |
75% Percentile (kb) | 228.3 | 237.5 | 306.4 | 192.4 | 168.1 |
Number of values measured | 189 | 130 | 118 | 172 | 140 |
Velocity of replication forks (kb/min) | |||||
Median (kb/min) | 2.37 | 2.45 | 2.48 | 1.84 | 1.16 |
Mean (kb/min) | 2.45 | 2.44 | 2.59 | 1.87 | 1.36 |
Minimum (kb/min) | 0.62 | 0.88 | 1.12 | 0.34 | 0.25 |
Maximum (kb/min) | 4.97 | 4.65 | 6.03 | 6.10 | 5.42 |
25% Percentile (kb/min) | 2.03 | 2.01 | 2.18 | 1.53 | 0.80 |
75% Percentile (kb/min) | 2.82 | 2.82 | 2.94 | 2.17 | 1.65 |
Number of values measured | 238 | 134 | 273 | 583 | 175 |
Asymmetry of replication forks (ratio long/short forks) | |||||
Median (ratio long/short forks) | 1.03 | 1.03 | 1.04 | 1.07 | 1.10 |
Mean (ratio long/short forks) | 1.07 | 1.12 | 1.08 | 1.19 | 1.22 |
Minimum (ratio long/short forks) | 1 | 1 | 1 | 1 | 1 |
Maximum (ratio long/short forks) | 2.03 | 2.34 | 1.56 | 3.77 | 3.13 |
25% Percentile (ratio long/short forks) | 1.00 | 1.01 | 1.01 | 1.02 | 1.04 |
75% Percentile (ratio long/short forks) | 1.08 | 1.10 | 1.10 | 1.18 | 1.24 |
Number of values measured | 104 | 72 | 85 | 112 | 126 |
Number of active replication origins/ haploid genome | |||||
Size of the haploid genome sequenced* | 32.4 Mb | 32.8 Mb | 32 Mb | 26 Mb | 2.8 103 Mb |
haploid genome/median IOD | 180 | 170 | 157 | 175 | 22.5 103 |
haploid genome/mean IOD | 172 | 168 | 142 | 163 | 20.6 103 |
