Figure 3 | Scientific Reports

Figure 3

From: Spines slow down dendritic chloride diffusion and affect short-term ionic plasticity of GABAergic inhibition

Figure 3

Spines slow down Cl− diffusion in dendrites imaged by live-cell super-resolution STED microscopy.

(A) STED image of spiny dendritic segment of a CA1 pyramidal cell. (B) The spatial variance of Cl− concentration in stochastic simulations of Cl− diffusion in STED-based dendritic morphologies. Note that the spatial variance in spiny dendrites is different from the spatial variance of idealized free (unhindered)Cl− diffusion (dotted line). (C) Dapp was determined from spatial variance (c.f. Fig. 1). The ratio of Dapp and DCl of 1 is characteristic for free (unhindered) Cl− diffusion. (D) In the presence of spines, the tortuosity was larger than 1 indicating a slower time course of longitudinal dendritic diffusion.

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