Figure 1
From: Micheliolide inhibits LPS-induced inflammatory response and protects mice from LPS challenge
MCL does not induce cellular apoptosis in Raw264.7.
(a) Chemical structure of MCL; (b) Raw264.7 cells (1.2 × 105) were plated overnight and stimulated by different concentrations of MCL with or without LPS (100 ng/mL) for 18 h. Cells were harvested and labeled with Annexin V and 7-AAD and analyzed by FACS. Similar results were obtained in three independent experiments. (c) Cell counting kit (CCK) cell proliferation assay. Raw264.7 cells (2 × 104/well) were seeded in 96-well plates. The culture medium was replaced with a medium containing MCL (0 μM, 5 μM or 10 μM). After 4 h of assay using the CCK, the optical density of each well was determined at 24 h, 48 h or 72 h (450/650 nm). Data are shown as mean ± SD of three independent experiments.
