Figure 5
Myosin-II activity is required for directional persistence but cell-level traction forces do not differ between FN and VN.
(A) REFWT DI and (B) cell speed on FN and VN (10 μg/ml) in presence of 0.1% DMSO, 5 μM Y-27632 or 25 μM blebbistatin are presented as box plots (middle line indicates the median, the cross the mean, the box the interquartile range, the whiskers the 5th and 95th percentiles). Data for control conditions are included and are the same as in Fig. 1. Selected columns were compared using unpaired t-tests. n: number of analyzed cells; Nexp: number of independent experiments. (C) Total traction force per cell calculated using traction force microscopy on FN- or VN-coated polyacrylamide substrates of two different elasticities (Young’s moduli of 6 or 12 kPa). An increase in traction force for the stiffer gels was observed but no significant differences between coatings. Experimental data on different coatings were compared using unpaired t-tests and between different elasticities using one-way ANOVA with Tukey post-test analysis (n.s.: not significant; ***p < 0.001).
