Table 1 Percentage of REFWT that exhibit polarized protrusions and their mean directionality index (DI) from single cell motility assays on different coatings and under different experimental conditions.
Substrate | Treatment | n1 (Nexp2) | % cells with polarized protrusions | DI |
|---|---|---|---|---|
FN (10 μg/ml) | – | 210 (4) | 61 | 0.63 |
FN (1 μg/ml) | – | 168 (3) | 49 | 0.28 |
VN (10 μg/ml) | – | 61 (2) | 0 | 0.23 |
FN (10 μg/ml) | Serum-free | 108 (2) | 0 | – |
FN (10 μg/ml) | DMSO 0.1% | 123 (2) | 36 | 0.56 |
FN (10 μg/ml) | Soluble α5β1 antagonist | 69 (2) | 0 | 0.41 |
FN (10 μg/ml) | Soluble αvβ3 antagonist | 131 (2) | 71 | 0.41 |
Patterned α5β1 | – | 41(2) | 0 | 0.23 |
Patterned α5β1 & αvβ3 | – | 75(2) | 0 | 0.18 |
