Figure 4: Calcium chelation inhibits EMT, Hif1-α signalling and DNA damage repair, induced by doxorubicin, hypoxia and ionizing radiation respectively, in Huh7 and HepG2 cells.
From: Regulation of Multi-drug Resistance in hepatocellular carcinoma cells is TRPC6/Calcium Dependent
(a) Expression of EMT associated proteins E-Cadherin, Claudin1 and Vimentin (HepG2 cells express extremely low Vimentin) with or without BAPTA-AM (10 μM). (b) Expression of Hif1-α under hypoxia, with or without BAPTA-AM (10 μM). (c) Expression of DNA damage repair proteins, p-ATM, p-ATR and p-H2A.X 2 h and 24 h after IR (10Gy), with or without BAPTA-AM (10 μM). (d) Comparison of p-H2A.X expressions 2 h and 24 h after IR (10Gy), with or without BAPTA-AM (10 μM).
