Figure 6: Phosphorylation of STAT3 (Tyr705) is blocked by intracellular calcium chelation and STAT3 inhibitor NSC74859 (100 μM) attenuates various MDR-related mechanisms in HCC cells.
From: Regulation of Multi-drug Resistance in hepatocellular carcinoma cells is TRPC6/Calcium Dependent
(a) Western blotting demonstrating the expression of p-STAT3 induced by doxorubicin (0.2 μg/mL) (Dox) for 24 h, hypoxia (1% O2) (Hypo) for 6 h and ionizing radiation (10 Gy) (IR) (2 h later), with or without BAPTA-AM (10 μM). (b) Western blotting demonstrating the expressions of Claudin1 and Vimentin after doxorubicin treatment, Hif1-α under hypoxia and p-H2A.X 2 h after ionizing radiation with or without NSC74859 (100 μM).
