Figure 5
Expression of SigP in the porX mutant.
(A) Western blot analysis of SigP in ATCC 33277 (WT), a porX-deficient mutant (KDP363) and the porX/porX+ complement strain (KDP372). Whole cell lysates from each strain corresponding to 5 μl of bacterial culture at OD595 1.0 were applied to SDS-PAGE and transferred onto a PVDF membrane, followed by immunodetection with anti-SigP antibody. (B) Quantitative real time RT-PCR experiments with total RNA extracted from exponentially growing bacterial cells (OD595 0.6) were performed.
