Figure 3: Early detection of plasma circulating LF and EF during cutaneous infection with the wild type 9602WT-lux strain.
From: In vivo dynamics of active edema and lethal factors during anthrax
Same experimental conditions as described in Fig. 2. For each infected mouse, blood was collected and LF (A), EF (B) and bacterial CFU (D) were quantified in the plasma. The LF/EF ratio (C) was calculated from the LF and EF values obtained for each individual mouse. (E) Percentage of mice for which the EF or LF value was above the detection threshold (see Table 1, the Materials and Methods and the Results sections); also shown is the combined percentage of mice for which the LF and/or EF values were positive and the percentage of bacteriaemic mice. Threshold values are represented as dotted lines (0.9 log10 for CFU, 0.4 ng per ml and 2.5 pg/ml for LF and EF respectively; see results section). Results are expressed as pg per ml; each dot represents an individual mouse and the bar represents the mean for each mouse population at each infection stage. The asterisks denote statistically significant differences (Mann-Whitney test); *p < 0.05; **p < 0.01; ***p < 0.001.
