Table 1 Evaluation of DDT toxicity in two DDT-susceptible transgenic lines (91-C-MDR49A and 91-C-MDR49B) and two DDT-resistant transgenic lines (91-R-MDR49A and 91-R-MDR49B) of Drosophila using a contact exposure to DDT (μg/vial).

From: Splice form variant and amino acid changes in MDR49 confers DDT resistance in transgenic Drosophila

Strain

LD50a (95% C.L.) b

χ2(df)

Slope ± SD

RR c

3 h

6 h

9 h

12 h

3 h

6 h

9 h

12 h

3 h

6 h

9 h

12 h

91-R-MDR49A

3,920.3 (2,868.2–5,562)

833.9 (423.9–1,616.4)

241.6 (80.7–719.3)

103.8 (68–154.1)

2.79 (2)

18.43 (2)

17.14 (2)

0.03 (2)

1.6 ± 0.1

1.4 ± 0.1

1.1 ± 0.1

1.4 ± 0.1

1.0 d 0.9 e 0.5 f 1.0 g

91-C-MDR49A

3,893.2 (1,963.2–8862)

971.2 (509.9–1,777.9)

489.1 (164.6–2,251.5)

105.9 (58.6–180.3)

1.9 ± 0.2

2.0 ± 0.1

1.2 ± 0.1

1.4 ± 0.1

91-R-MDR49B

>50,000

11,834.8 * (9,603–16,025)

2,986.9 * (1,188–5968.5)

600.7 * (408–996.6)

–

131.1 (2)

101.1 (2)

36.4 (2)

–

2.1 ± 0.3

2.8 ± 0.2

2.0 ± 0.2

3.2 e 3.0 f 2.1 g

91-C-MDR49B

>50,000

3,284.4 * (1,481–6,398.7)

1,002.3 * (438.7–2,192.1)

299.9 * (185.6–501.8)

–

2.6 ± 0.2

2.7 ± 0.2

3.0 ± 0.3

Strain

LD 50 a (95% C.L.) b

χ2 (df)

Slope ± SD

RR c

15 h

18 h

21 h

24 h

15 h

18 h

21 h

24 h

15 h

18 h

21 h

24 h

91-R-MDR49A

39.8 (31.4–50.2)

17.1 (10.9–28.9)

12.2 (7.5–22.7)

7.8 (4.9–14.7)

1.86 (2)

5.1 (2)

3.84 (2)

9.54 (2)

1.7 ± 0.1

2.4 ± 0.2

2.7 ± 0.2

2.6 ± 0.2

1.2 h 1.1 i 1.0 j 1.4 k

91-C-MDR49A

32.3 (12.1–81.7)

15.1 (11.8–19.6)

11.9 (8.4–17.9)

5.7 (5.0–6.6)

1.8 ± 0.1

3.0 ± 0.2

3.3 ± 0.3

2.8 ± 0.2

91R-MDR49B

68.1 (40.5–115.1)

46.2 (40.1–53.2)

35.9 (21.1–58.6)

18.6 (11.9– 29.8)

3.34 (2)

5.19 (2)

1.66 (2)

5.18 (2)

2.8 ± 0.2

3.2 ± 0.3

3.1 ± 0.3

3.7 ± 0.4

1.2 h 1.3 i 1.1 j 1.2 k

91-C-MDR49B

56.8 (37.2–86.2)

36.7 (32.1–42.1)

29.9 (18.4–48.4)

17.9 (11.4–28.7)

2.4 ± 0.2

3.4 ± 0.3

2.9 ± 0.2

3.4 ± 0.3

  1. aLethal Dose (ug/vial) that killed 50% of the flies.
  2. b95% Confidence limit.
  3. cResistance ratio = 91-R LD50/91-C LD50.
  4. dResistance ratio at 3 h DDT treatment.
  5. eResistance ratio at 6 h DDT treatment.
  6. fResistance ratio at 9 h DDT treatment.
  7. gResistance ratio at 12 h DDT treatment.
  8. hResistance ratio at 15 h DDT treatment.
  9. iResistance ratio at 18 h DDT treatment.
  10. jResistance ratio at 21 h DDT treatment.
  11. kResistance ratio at 24 h DDT treatment.
  12. *Log DDT dose versus mortality regressions of 91-R-MDR49A versus 91-C-MDR49A and 91-R-MDR49B versus 91-C-MDR49B flies were significantly different using the maximum log-likelihood ratio test, respectively (p < 0.05).
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