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Figure 1

From: More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments

Figure 1

Response surface model for biomass accumulation.

Three-dimensional response surface model graphs showing the impact of temperature (A, °C), inoculum density (C, % (v/v)) and incubation time (B, days) on the fresh cell weight yield (FW, grams) of the pear cell suspension culture (50 ml culture volume). Upper row: Fresh cell weight yield shown as a function of incubation temperature (B) and incubation time (A), while the inoculum density is set constant at three different levels: 10%, 20% and 30%. Lower row: Fresh cell weight yield shown as a function of inoculum density (C) and incubation time (A), while the incubation time is set constant at three different levels: 20 °C, 24 °C and 30 °C. All cells were grown under illumination (16-h photoperiod).

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