Table 2 Factors and factor interactions used to predict biomass accumulation.

From: More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments

Factor

F-value

p-value

Model

89.30

<0.0001

A (temperature)

1.91

0.1810

B (incubation time)

59.86

<0.0001

C (inoculum density)

126.92

<0.0001

D (light)

561.77

<0.0001

AD

10.03

0.0045

BD

5.69

0.0262

CD

37.95

<0.0001

A2

26.65

<0.0001

B2

6.40

0.0191

C2

40.02

<0.0001

  1. A reduced quadratic model was used to analyze the data. Factors showing a significant influence on the biomass yield were preselected by automated backward selection with a p-value threshold of 0.100. Factors with a p-value > 0.05 were removed manually, except those needed to maintain the model hierarchy. A p-value of 0.05 indicates a significance (alpha) level of 5%.