Table 4 Factors and factor interactions used to predict the accumulation of PSL.

From: More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments

Factor

F-value

p-value

Model

24.06

<0.0001

A (temperature)

30.61

<0.0001

B (incubation time)

7.25

0.0130

C (inoculum density)

23.93

<0.0001

D (light)

73.87

<0.0001

AB

23.56

<0.0001

AC

4.39

0.0473

AD

25.68

<0.0001

BC

6.74

0.0161

BD

5.59

0.0268

  1. A reduced two-factor interaction model was used to analyze the data. Factors showing a significant influence on the amount of PSL were preselected by automated backward selection with a p-value threshold of 0.100. Factors with a p-value > 0.05 were removed manually, except those needed to maintain the model hierarchy. A p-value of 0.05 indicates a significance (alpha) level of 5%.