Table 2 Calibration data for each standard ecdysteroid.

From: An unexpected advantage of insectivorism: insect moulting hormones ingested by song birds affect their ticks

Compound

Regression equation

R2

Linear range (ng/mL)

LODa (ng/mL)

LOQb (ng/mL)

20E

y = 59.27x + 44.0

0.9988

1.40–1428.6

0.24

0.79

pB

y = 36.61x + 19.1

0.9998

1.40–1428.6

0.20

0.66

pS

y = 78.44x + 28.3

0.9998

1.40–1428.6

0.34

1.12

E

y = 67.86x + 22.0

0.9995

5.58–1428.6

0.77

2.56

2d20E

y = 98.14x + 66.2

0.9996

1.40–1428.6

0.27

0.89

ajC

y = 32.42x–47.7

0.9999

1.40–1428.6

0.38

1.27

Ds

y = 134.57x + 26.8

0.9995

0.35–1428.6

0.14

0.47

  1. Abbreviations: 20-hydroxyecdysone (20E), polypodine B (pB), poststerone (pS), ecdysone (E), 2-deoxy-20-hydroxyecdysone (2d20E), ajugasterone C (ajC), dacryhainansterone (Ds). aLOD: limit of detection.
  2. bLOQ: limit of quantification.
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