Figure 3
From: Biological insertion of computationally designed short transmembrane segments
Predicted and experimental pi values of sequences including the most prevalent amino acid residues in TM helices.
Upper panel: Computationally designed leucine and alanine sequences of different lengths (l). The predicted values for each given sequence are shown in blue and the measured values obtained for three independent experiments in orange. The gray area represents the predictions of the pi values for the 500 sequences between percentiles 0.25 and 0.75 of the total population (1,000 computed sequences, see Fig. S2). Central panel: Similar to upper but the computationally designed TM sequences contained leucine, alanine, valine, isoleucine, glycine and phenylalanine. Bottom panel: Similar to upper but including, in addition to the hydrophobic, the more prevalent polar and aromatic residues in TM segments. See Supplementary Information Table 2 for details on the TM-tested sequences used.
