Figure 2: AHNAK ablation induces the transition of white to brown fat and enhances thermogenesis. | Scientific Reports

Figure 2: AHNAK ablation induces the transition of white to brown fat and enhances thermogenesis.

From: AHNAK deficiency promotes browning and lipolysis in mice via increased responsiveness to β-adrenergic signalling

Figure 2

(A) AHNAK mRNA expression of fat depots in control or CL-treated mice (n = 4–9 per group); expression was normalized to that of 36B4. (B) Representative images of H&E staining in various adipose tissues. (C) Anti-UCP1 (green) immunofluorescence staining of adipose tissues treated or not with CL CL-316243. (D) mRNA expression of thermogenic genes in eWAT and iWAT analysed by qPCR (n = 5). (E,F) Protein expression of thermogenic genes in response to CL-316243 injection in eWAT (E) and iWAT (F) analysed by immunoblotting; GAPDH was used as a loading control. (G) Core body temperature measured in WT and KO mice after CL injection at room temperature (n = 12). (H,I) VO2 (H) and heat generation (I) analysed by indirect calorimetry in WT and KO mice (n = 7 per group). (J,K) Immunoblotting analysis of mitochondrial respiratory chain complexes in eWAT (J) and iWAT (K) GAPDH was used as a loading control. The data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, wild-type (WT) versus Ahnak−/− (KO) mice.

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