Figure 3: Increased levels of eosinophils and type 2 cytokines in AHANK-deficient WAT. | Scientific Reports

Figure 3: Increased levels of eosinophils and type 2 cytokines in AHANK-deficient WAT.

From: AHNAK deficiency promotes browning and lipolysis in mice via increased responsiveness to β-adrenergic signalling

Figure 3

(A) Gating strategy for identification of eosinophil lineage defined as CD45+ CD11b+ Siglec F+. (B,C) Representative plots and frequencies (B) and number (C) of eosinophils in eWAT of mice treated with CL-316243 (n = 4 group pooled from 7–10 mice). Statistical analysis was performed using the Kruskal-Wallis test (***P < 0.001) (D–F) qPCR analysis of eosinophil- and type 2 cytokine-related genes in eWAT (D), iWAT (E), and BAT (F) of mice treated with CL-316243 (n = 5–7); expression was normalized to that of 36B4. Data are the mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001.

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