Figure 2 | Scientific Reports

Figure 2

From: Nuquantus: Machine learning software for the characterization and quantification of cell nuclei in complex immunofluorescent tissue images

Figure 2

Immunofluorescent staining of cardiac tissue post myocardial infarction (MI).

Top panels: mouse MI cardiac left ventricular tissue, bottom panels: swine MI cardiac left ventricular tissue. (a) Viable zone (VZ) (also known as remote zone) represents the tissue that is distant from the ischemic injury and contains morphologically normal CMs. (b) Border zone (BZ) represents the transition from injury site to surviving CMs. The surviving CMs in the swine BZ are localized in adjacent form of islands whereas in mouse the CMs are equally distributed along the BZ. (c) Infarct Area (IA) represents the site of ischemic injury that is characterized by CMs loss and formation of scar tissue. Due to differences in cardiac anatomy and MI surgical procedures, the swine IA is very large and contains interstitial cell nuclei without any CMs. On the contrary, the mouse contains few surviving CMs, which are outnumbered by the interstitial cell nuclei. Blue: nuclei staining via 4′, 6-diamidino-2-phenylindole (DAPI). Red: cardiac specific structural protein staining for α-sarcomeric actin. Examples for CM nuclei are shown via white arrows and are identified if they contain DAPI positive staining and surrounded by α-sarcomeric actin positive staining. Examples for non-CMs DAPI positive nuclei are shown via yellow arrows. Scale 25 μm.

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