Table 1 Purification of ACS from D. tertiolecta.

From: Characterization and expression of AMP-forming Acetyl-CoA Synthetase from Dunaliella tertiolecta and its response to nitrogen starvation stress

Purification step

Protein

Activity (U)

Specific activity

Yield (%)

Purification

(mg)

(U/mg)

(-fold)

Crude extract

305.200

270.102

0.885

100

1.0

After purification

3.717

211.575

56.921

78.33

64.3

After ultrafiltration

2.874

151.957

52.873

56.26

59.7

  1. Enzyme activity was measured at 37 °C for 5 min as described in Materials and methods. 1 U = 1 μmol NADH·min−1.
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