Figure 2
From: Lighting Up Clostridium Difficile: Reporting Gene Expression Using Fluorescent Lov Domains
Expression of phiLOV in different Clostridium species.
Comparison of the relative fluorescent intensities observed between plasmid-free C. difficile R20291 (CD WT), C. sordellii ATCC9714 (CS WT) & C. acetobutylicum ATCC824 (CA WT) and these strains expressing pRPF185-phiLOV. For this experiment, each Clostridium species were grown to A600 nm of 0.5 and induced by the addition of 500 ng/ml ATc for 60 min. Results shown are mean ± standard error of mean (SEM) of all bacterial cells from five de-convoluted fields of view and a minimum of two independent experiments.
