Figure 4: Impaired IFN-γ production by adipose tissue stromal vascular fraction cells isolated from infected IL-12/IL-23p40−/− mice. | Scientific Reports

Figure 4: Impaired IFN-γ production by adipose tissue stromal vascular fraction cells isolated from infected IL-12/IL-23p40−/− mice.

From: Enrichment of IFN-γ producing cells in different murine adipose tissue depots upon infection with an apicomplexan parasite

Figure 4

(a) IFN-γ levels in the supernatants of mesenteric or subcutaneous adipose tissue (MAT and SAT, respectively) SVF cells cultured for 48 h alone (unstimulated) or in the presence of freeze-killed NcT (stimulated) recovered from control (PBS) or N. caninum-infected (NcT) wild type (WT) or IL-12/IL-23p40−/− (p40−/−) C57BL/6 mice, as indicated, 24 h after intraperitoneal (i.p.) challenge. Each symbol represents an individual mouse. Bars represent means of 6 mice per group pooled from 2 independent experiments. (Mann-Whitney U, *P < 0.05; **P ≤ 0.01). (b) Representative images showing parasitic forms closely associated with stromal vascular fraction cells isolated from the MAT and SAT of WT or p40−/− mice 24 h after i.p. administration of 1 × 107N. caninum tachyzoites, detected by immunohistochemistry. Cells were specific stained (brown coloration) with a polyclonal serum goat anti-N. caninum and counterstained with haematoxylin. This is one representative result of 2 independent experiments with 3 mice per group per experiment. Bar = 20 μm.

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