Figure 2: MPHh+ induce a pro-osteogenic program in human MSCs.
From: Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway
(a) MPHh+ inhibit adipocyte differentiation of human mesenchymal stem cells (hMSCs). hMSCs were placed in adipogenic conditions and incubated with 10 μg/mL of MPHh+ or MPHh−. Represented images of ORO-stained cells are shown, n = 2 independent experiments (scale bar: 50 μm). (b) MPHh+ alter impair protein expression of key adipocyte factors of hMSCs-differentiated adipocytes. Unprocessed immunoblots, from which the presented images were cropped, are presented Fig. S7. (c,d) MPHh+ induce the expression of osteogenic markers in hMSCs. hMSCs were incubated with 10 μg/mL MPHh+ in adipogenic conditions. mRNA expression of transcription factor Runx2 was assessed by real-time qPCR (c) and alkaline phosphatase activity (ALP) was evaluated following ALP staining assay (d). Representative images of ALP-stained cells are shown (scale bar: 50 μm).
