Figure 4: LDE225 Smo antagonist reverses adipogenesis blockade in MPHh+ and GSA-10-treated 3T3-L1 cells.
From: Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway
(a,b) Effects of Smo antagonists (Cyclopamine, 10 μM; GDC-0449, 3 μM; SANT-1, 1 μM; MRT-92, 1 μM; LDE225, 3 μM) added to 3T3-L1 cells concomitantly incubated with SAG, recShh, MPHh+ and GSA-10 (at similar concentrations to those used for Fig. 3a) in minimal induction conditions were compared to vehicle-treated 3T3-L1 (no antagonist). Representative ORO-stained images of these experiments are presented in (a) and ORO-quantification results (b) are presented as dots with median plus range from n = 3–5 independent experiments.
