Figure 1: Single-crossover recombination process. | Scientific Reports

Figure 1: Single-crossover recombination process.

From: In vitro template-change PCR to create single crossover libraries: a case study with B. thuringiensis Cry2A toxins

Figure 1

Only the forward recombination process is shown. The small triangles represent ddATP molecules, and the gray regions in primers or DNA fragments represent flanking sequences used for selective amplification. (A) Generation of reverse ssDNA templates from cry2Aa (using primers F and R) and cry2Ad (using primers F’ and LR) by asymmetric PCR. (B) Generation of randomly terminated cry2Aa forward chains using primer LF. (C) Re-annealing and extension of cry2Aa forward chains using the cry2Ad ssDNA template to generate chimeric double-stranded DNA amplicons. (D) Use of the flanking sequence primers (SF and SR) for selective amplification and resolution of the chimeras.

Back to article page