Figure 2: Crossover regions of the generated chimeras relative to a cry2Aa-cry2Ad gene alignment. | Scientific Reports

Figure 2: Crossover regions of the generated chimeras relative to a cry2Aa-cry2Ad gene alignment.

From: In vitro template-change PCR to create single crossover libraries: a case study with B. thuringiensis Cry2A toxins

Figure 2

The crossover occurred in a region containing identical DNA sequences between cry2Aa and cry2Ad, and these are indicated by open rectangles. 2Aa and 2Ad refer to the cry2Aa and cry2Ad genes respectively. R01 to R37 are the crossover regions of chimeras, the numbers in parentheses means the number of sequenced chimeras with crossovers within that region. Regions of sequence identity are indicated by gray shading and the vertical lines represent the boundaries between the three structural domains.

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