Figure 3
(a–c) Quantitative tracking results of head angle, flagellar beating asymmetry and flagellar beating amplitude of single sperm. A freely swimming control sperm and three representative sperm during rheotaxis reorientation are shown. Fluid flow was turned on at time 0 and kept constant afterwards. (d,e) Motion history images confirm no active asymmetry flagellum bending for both control sperm under the no-flow condition and rheotaxis sperm. Scale bar: 10 μm. (f–i) Data from a sperm population. n = 15–26 sperm for each data point. Sperm turning process is described by four phases: head angle at 90°, 60°, 30° and 0°. Box plots and mean values of flagellar beating asymmetry (f,g) and beating amplitude (h,i) are shown.
