Figure 3: Overexpression of WIP overcomes WIRE deficiency. | Scientific Reports

Figure 3: Overexpression of WIP overcomes WIRE deficiency.

From: WIP and WICH/WIRE co-ordinately control invadopodium formation and maturation in human breast cancer cell invasion

Figure 3

(a) WIP, WIRE and GAPDH levels were analyzed by WB in shControl, shWIP or shWIRE soluble MDA-MB-231 cell lysates (left). Quantification of WB experiments is shown (right). (b) Soluble lysates from MDA-MB-231 transiently overexpressing eGFP or WIP-eGFP, WIP and WIRE levels were analyzed by WB (left) and relative expression was quantified using GAPDH expression as a control (right). (c) Cells overexpressing Flag-WIRE or Flag-WIP in WIP- and WIRE-deficient cells were allowed to invade rhodamine-gelatin (red, 5 h), fixed in 4% PFA, and stained for F-actin (green) and cortactin (cyan). Bars: 10 μm. (d) Quantification of relative invadopodium formation (number of cells that form invadopodia) and degradation (number of cells that degrade gelatin) normalized to control values. Data show mean ± SD of at least three independent experiments (N ≥ 70 cells/condition). *p < 0.05, **p < 0.01, ***p < 0.001 by 1-way ANOVA and Tukey’s post-hoc test (a), Student’s t-test (b) or Chi-square test (d).

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