Figure 4: Inhibition of N-WASP activation mimics the lack of WIRE. | Scientific Reports

Figure 4: Inhibition of N-WASP activation mimics the lack of WIRE.

From: WIP and WICH/WIRE co-ordinately control invadopodium formation and maturation in human breast cancer cell invasion

Figure 4

shControl, shWIP and shWIRE MDA-MB-231 cells were allowed to invade rhodamine-gelatin (red) in the presence of vehicle or 2 μM wiskostatin (3 h). (a) Cells were fixed and stained for F-actin (green) and cortactin (cyan). Bars: 10 μm (insets, 5 μm). (b) Quantification of relative invadopodium-forming cells (number of cells that form invadopodia) and degrading cells (number of cells that degrade gelatin) normalized to shControl values. Data show mean ± SD of at least three independent experiments (N ≥ 98 cells/condition). *p < 0.05, **p < 0.01, ***p < 0.001, Chi-square test. (c) Orthogonal views of shControl (treated or not with wiskostatin), shWIP and shWIRE cells shown in (a); arrows indicate invadopodia (F-actin- and cortactin-positive protrusions).

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