Figure 4
Male Mito-Ob mice with HCC exhibit mitochondrial dysregulation and increased hepatic oxidative DNA damage.
(a) Representative photomicrographs showing reduction in mitochondria content in the hepatic lesion as determined by immunohistochemistry using anti-prohibitin antibody. Magnification 10X. Magnified view of hepatocytes is shown in the insets. (b) Left panel: Representative immunoblots showing expression level of mitochondrial marker proteins in the liver from Mito-Ob mice and control mice. Nrf-2 was used as a control for nuclear transcription factor and beta-tubulin blot is shown as a loading control. Similar conditions were used for the electrophoretic analysis of proteins by Western immunoblotting. Right panel: Histograms showing quantification of protein levels. Data are presented as mean ± SEM (n = 5–7 mice in each group). (c) Representative photomicrographs showing oxidative DNA damage in the liver at 12–14 months of age. (d) Histograms showing quantification of oxidative DNA damage in the liver as shown in the panel (c). (e) Histograms showing mitochondrial copy numbers as determined real time-PCR. Data are presented as mean ± SEM (n = 5–7 mice in each group). Asterisks indicate comparison between sex matched Mito-Ob vs Wt. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test. Diamonds indicate comparison between Tu and No Tu group by Dunnett’s t test. Wt – wild type; Tu – tumor; No Tu – No tumor; MW – molecular weight; KD – kilo Dalton.
