Figure 5
ERK1/2 and STAT3 signaling are inversely altered in the liver tumors from Mito-Ob mice.
(a) Left panel: Representative immunoblots showing liver ghrelin level at 12–14 months of age as determined by rabbit polyclonal anti-ghrelin antibody. Data from two different animals in each group is shown. Similar conditions were used for the electrophoretic analysis of proteins by Western immunoblotting. Right panel: Histogram showing quantification of the liver ghrelin level as shown in the left panel. Data are presented as mean ± SEM (n = 3–4 mice in each group). Asterisks indicate comparison between sex matched Mito-Ob vs Wt. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test. Diamonds indicate comparison between Tu and No Tu group, square indicate comparison between Tu and Fem and circle indicate comparison between No Tu and Fem by Dunnett’s t test. (b) Left panel: Representative immunoblots showing the activation level of Akt, ERK and STAT3 signaling pathways in the liver at 12–14 months of age as determined by their phospho-specific antibodies. Similar conditions were used for the electrophoretic analysis of proteins by Western immunoblotting. Right panel: Histograms showing quantification of the activation level of Akt, ERK and STAT3 in the liver as shown in the left panel. Data are presented as mean ± SEM (n = 5–7 mice in each group). Asterisks indicate comparison between sex matched Mito-Ob vs Wt. NS, not significant; *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test. Diamonds indicate comparison between Tu and No Tu group by Dunnett’s t test. ♦P < 0.05. Wt – wild type; Tu – tumor; No Tu – No tumor; Fem – female; MW – molecular weight; KD – kilo Dalton.
