Figure 4
From: Morphotype-specific effector functions of Cryptococcus neoformans PUM1
Pum1 modulates the transcript abundance and stability of ZNF2.
(a) Photomicrographs (DIC) of the PGAL7ZNF2 and PGAL7ZNF2 pum1∆ strains induced for 3 hours. (b) Northern analysis of either ZNF2 or GAL7 expression in PGAL7ZNF2 and PGAL7ZNF2 pum1∆ strains grown to mid-log phase in YPD or induced (YP-GAL) for 3 hours. (c) Northern analysis of ZNF2 stability in the PGAL7ZNF2 and PGAL7ZNF2 pum1∆ strains after transcriptional shutoff (YPD). The hybridization level of the ZNF2 was normalized to the rRNA levels to determine the change in transcript stability over time.
