Figure 2: Luciferase assay to analyze transcriptional activity. | Scientific Reports

Figure 2: Luciferase assay to analyze transcriptional activity.

From: Mild decrease in TBX20 promoter activity is a potentially protective factor against congenital heart defects in the Han Chinese population

Figure 2

(a) Promoter activity analysis using dual-reporter luciferase system in three cell lines. P6-Major contains all major alleles of six positive SNPs, and P6-Minor contains all six minor alleles. Luciferase assay showed that P6-Minor has decreased transcriptional activity compared with major alleles in three different cell lines. (n > 3, *p < 0.05, **p < 0.01, compared with P6-Major); (b) Promoter activity analysis of four haplotypes using dual-luciferase reporter system in three cell lines (n > 3, *p < 0.05, **p < 0.01, compared with Haplotype AAT in the same cell line).

Back to article page