Figure 2: Tmem176a and b mRNA expression in intestinal ILC3s.
From: RORγt+ cells selectively express redundant cation channels linked to the Golgi apparatus
(a) Lymphocytes from intestinal lamina propria of RORγt-fate map mice (Rorc(γt)-CreTG × Rosa26-tdRFP) were isolated. Lineage-negative (CD11b−CD11c−CD19−TCRαβ−TCRγδ−) RFP+NK1.1− ILC3-enriched and RFP−NK1.1+ ILC1s were FACS-sorted. In parallel, CD11b/c+ and lineage-negative NK1.1+ conventional NK/ILC1s were FACS-sorted from the spleen. (b) Expression of indicated genes (mean ± SD) was assessed by quantitative RT-PCR in each population isolated from 3–4 independent mice. Statistically significant differences between ILC3s and the other populations are indicated: *p < 0.05, **p < 0.01, ***p < 0.001.
