Figure 6: Tmem176b single-deficient mouse susceptibility to the development of psoriasis-like dermatitis.

(a) Psoriasis-like dermatitis was induced in WT mice by topical application of imiquimod (IMQ) cream on the shaved back skin. At day 4, draining (inguinal) lymph nodes were harvested and TCRγδ⁺ and TCRβ⁺CD4⁺ T cells were FACS-sorted. (b) Expression of indicated genes was assessed by quantitative RT-PCR. Each dot represents an individual mouse (n = 6 in each group). Statistically significant differences are indicated: **p < 0.01. (c) Psoriasis-like dermatitis was induced in WT (n = 27) and Tmem176b^−/− (n = 28) mice by topical application of imiquimod (IMQ) cream on the shaved back skin during 5 consecutive days. Mice were weighed and scored daily on a scale from 0 to 4 for skin thickening, erythema and scaling. *p < 0.05, ***p < 0.01. (d) Expression of indicated genes was assessed by quantitative RT-PCR in the skin of untreated or IMQ-treated WT and Tmem176b^−/− mice (n = 4–6 in each group). Statistically significant differences between IMQ-treated WT and Tmem176b^−/− mice are indicated: *p < 0.05.