Figure 1 | Scientific Reports

Figure 1

From: Human cytomegalovirus gH stability and trafficking are regulated by ER-associated degradation and transmembrane architecture

Figure 1

gH is localized within the cell during a CMV infection.

(A) MRC5 fibroblasts infected with the CMV reporter strain AD169IE2-YFP (MOI: 5) were harvested between 48 and 96 hpi and analyzed by flow cytometry for YFP expression (x-axis) and gH expression (y-axis). Cells were either untreated (top row) or permeabilized with saponin (bottom row) to distinguish between extracellular versus intracellular gH protein. Percent of double-positive (YFP+, gH+) cells is indicated in the top right panel. (B) Percent of YFP + cells that were positive for gH was determined for non permeabilized (top panel) and permeabilized (bottom panel) samples from all time points.

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