Figure 1: Forced SIRPα expression inhibited proliferation and caused apoptosis in APL and hepatocellular carcinoma cell line. | Scientific Reports

Figure 1: Forced SIRPα expression inhibited proliferation and caused apoptosis in APL and hepatocellular carcinoma cell line.

From: Role of Signal Regulatory Protein α in Arsenic Trioxide-induced Promyelocytic Leukemia Cell Apoptosis

Figure 1

(a) Western blotting of SIRPα level in HL-60 and Huh7 cells 48 h post-infection with SIRPα overexpression lentivirus, LV-SIRPα and control lentivirus, LV-CTL. (b) Growth curves of HL-60 and Huh7 cells infected with LV-SIRPα or LV-CTL. MTT assays were used to measure cell proliferation on days 1, 2, 3 post-infection. Absorbance was measured at 492 nm. (c) Western blotting of cleaved caspase-3 level in HL-60 and Huh7 cells infected with LV-SIRPα or LV-CTL on the third day post infection. (d) Flow cytometry analysis of apoptosis of HL-60 and Huh7 cells infected with LV-SIRPα or LV-CTL on the third day post infection with annexin V-PI staining: representative flow cytometer results (left panel) and quantitative analysis of apoptosis (right panel). Values were shown as the mean ± SEM (n = 3). *P < 0.05. **P < 0.01.

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